CSTM-g-PEG-FA nanoparticles for gene delivery / 中国药学杂志

ABSTRACT

OBJECTIVE: To synthesize a water-soluble cationic polymer of quatemized chitosan modified by folic-acid for active targeting and to study its role as a gene vector. METHODS: Folic-acid was connected to quatemized chitosan by bi-functional PEG, CSTM-g-PEG-FA/pDNA nanoparticles were prepared by self-assembly, and the gene transfection efficiency mediated by the nanoparticles into 293T cells in vitro was observed. RESULTS: When weight ratio of CSTM-g-PEG-FA/pDNA was 5 or greater than 5, pDNA was completely wrapped. And the transfection efficiency of CSTM-g-PEG-FA/pDNA nanoparticles was significantly higher than CSTM/pDNA nanoparticles. CONCLUSION: Cationic polymer CSTM-g-PEG-FA can electrostatically bind and condense anionic DNA into nanometersized particles and has the function of active targeting.