Protective effect of gastrodin preconditioning against myocardial ischemia reperfusion injury in rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To observe the effect of gastrodin preconditioning on the myocardial ischemia reperfusion injury (MIRI) in rats and to study the mechanism. METHODS: SD rats were randomized into six groups including sham group, NS group, gastrodin groups of low middle and high concentrations and verapamil group. The rats were treated with gastrodin via intragastric administration for one week. Then the rats were treated by closing the left anterior descending coronary artery for 30 min and then releasing it for 2 h under anesthesia. The changes of the arrhythmia, myocardial histological structure and serological tests including SOD, LDH, CK-MB and MDA were examined. Furthermore, the effect of gastrodin on store-operated Ca2+ influx was also investigated. RESULTS: It was found that gastrodin significantly enhanced SOD production and suppressed the incidences of arrhythmia and LDH, CK-MB and MDA concentrations. Additionally, gastrodin inhibited store-operated Ca2+ influx. CONCLUSION: Gastrodin has protective effect on myocardium against ischemia reperfusion injury in rats through several pathways including cleaning oxygen radicals and reducing the overload of Ca2+.