Uncertainty evaluation of determination of aflatoxin B1 in Zizyphi Spinosi Semen by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an uncertainty analysis method for determination of aflatoxin B1 in Zizyphi Spinosi Semen by HPLC. METHODS: The uncertainty arising from various factors in the whole process of analysis was evaluated. The facilitated method of evaluation was achieved by analysis of the uncertainty sources arising from the procedure of analysis and by using the validation data of assay. All the standard uncertainties were then combined according to the appropriate rules to give a combined standard uncertainty and an expanded standard uncertainty. RESULTS: When the residue level of aflatoxin B1 in Zizyphi Spinosi Semen was 6.218 μg · kg-1, the expanded standard uncertainty for aflatoxin B1 was 0.496 μg · kg-1(P=95%). CONCLUSION: This method is applicable for the uncertainty evaluation of aflatoxin B1 residue determination. It provides an important reference for the uncertainty evaluation for the aflatoxin B1 residue determination in Zizyphi Spinosi Semen.