Simultaneous determination of five phenolic acids in Salviae Miltiorrhizae Radix et Rhizoma by HPCE / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an high performance capillary electrophoresis (HPCE) method for simultaneous determination of rosmarinic acid, protocatechualdehyde, danshensu, salvanic acid B, and salvianolic acid A in Salviae Miltiorrhizae Radix et Rhizoma. And to determine the optimum drying temperature for the herb. METHODS: 10 mmol · L-1 boric acid -12 mmol · L-1 monosodium phosphate-10 mmol · L-1 SDS was used as buffer solution, an uncoated fused silica capillary column (75 μm × 64.5 cm, 56 cm of effective length) was used, the separation voltage was 18 kV, detection wavelength was 206 nm, column temperature was maintained at 20°C, and sample was injected at 5 kPa × 6 s. RESULTS: Five components showed good linearity (r>0.9990) in the ranges of the tested concentrations. The optimum drying temperature was 100°C. CONCLUSION: The method is simple, accurate and reproducible, and can be used for the quality control of Salviae Miltiorrhizae Radix et Rhizoma.