Study on HPTLC chromatographic fingerprint analysis of Ilex pubescens and its related species / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop the high-performance thin-layer chromatographic (HPTLC) fingerprints of triterpenoid from Ilex pubescens and compare the fingerprints similarity with its related species, such as Ilex rotunda and Ilex asprella. METHODS: The separation was performed on pre-coated HPTLC GF254 silica-gel plate stationary phase with trichloromethane-ethyl acetate-methanol-water-formic acid(3:22:5:2:0.2) as solvent system and 10% sulfuric acid alcoholic solution as color development reagent. The common pattern of HPTLC fingerprints were obtained through Chromafinger2005 solution software, and authentication and quality assessment were analyzed by similarity and Principle Commonent Analysis. RESULTS: The common pattern of the pileus of Ilex pubescens consists of 13 characteristic peaks. There are notable differences among the fingerprints of different parts of Ilex pubescen, and those between Ilex pubescens and its related species. CONCLUSION: The different parts of Ilex pubescens can be distinguished by comparison of the HPTLC chromatographs. The other relatives can be easily differentiated from Ilex pubescens too. Copyright 2012 by the Chinese Pharmaceutical Association.