Cinnamaldehyde ofloxacin-3-ylhydrazone induces apoptosis of human pancreatic carcinoma cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the effect of cinnamaldehyde ofloxaein-3-ylhydrazone (FQ)16) on apoptosis of human pancreatic carcinoma cells in vitro. METHODS: Human pancreatic carcinoma cell line BxPC-3 was treated with FQ16 at different concentrations. The proliferation inhibition was examined by MTT assay. Morphological examination, TUNEF and agarose gel electrophoresis method were used to detect apoptosis. Western blotting method was used to detect the expression of apoptosis related genes caspase-3 , caspase-9, caspase-8, bcl-2, bax and cytochrome C. RESULTS: The cell proliferation was inhibited by FQ16 in a time- and a dose-dependent manner. Treatment of BxPC-3 cells with different concentrations of FQ16 for 24 h increased the percentage of the apoptotic cells obviously (P < 0.05); the morphology of BxPC-3 showed changes such as nuclear chromatin condensation and fragmentation and typical ladder DNA in apoptotic cells. FQ16 increased protein expression of bax, caspase-9 and caspase-3, and induced cytosolic accumulation of active caspase-9 and caspase-8, whereas the protein expression of bcl-2 decreased. Western blotting results reveal that FQ16 released mitochondrial cytochrome C into cytosol in a dose-dependent manner. CONCLUSION: Apoptosis in pancreatic carcinoma BxPC-3 cells is one of the key mechanisms of action of cinnamaldehyde olloxacin-3-ylhydrazone, and mitochondrial-dependent pathways are involved in the induction of apoptosis of BxPC-3 cells. Copyright 2012 by the Chinese Pharmaceutical Association.