Study on HPLC fingerprints of flavone C-glycosides in Dendrobium officinale leaves and determination of index component / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the HPLC fingerprints of flavone C-glycosides in Dendrobium officinale leaves and determine the content of apigenin-6-C-α-L-arabinoside-8-C-β-D-xyloside for the quality control. METHODS: The compounds were separated by using XB C18(4.6 mm × 250 mm, 5 μm) analytical column. The mobile phase consisted of methanol and water containing 0.4% formic acid. Gradient elution program was used. The detection wavelength was 335 nm. In total 14 batches of Dendrobium officinale leaves and 3 batches of different species from Dendrobium were analyzed. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004AB) and principal component analysis(PCA) were used in data analysis. RESULTS: The HPLC fingerprint of flavone C-glycosides of Dendrobium officinale leaves was established. In total 9 peaks were selected as the characteristic common peaks and 8 of them were identified. There were significant differences in the fingerprint chromatograms between Dendrobium officinale leaves and different species from Dendrobium. Principal component analysis showed that apigenin-6-C-α-L-arabinoside-8-C-β-D-xyloside among the flavone di-C-glycosides was the most important component. CONCLUSION The HPLC fingerprint and content of major component can be applied for identification and quality control of Dendrobium officinale leaves.