Determination of six active components in Zhikechuanbeipipa dropping pills by UPLC-Q-TOF / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a UPLC-Q-TOF-MS method for determination of six components (guanosine, peiminine, platycodin D, platycodin D3, oleanolic acid and ursolic acid) in Zhikechuanbeipipa dropping pills. METHODS: The analysis was performed on a Waters Acquity BEH C18 column (2.1 mm×100 mm, 1.7 μm) with the mixture of acetonitrile-water-formic acid as mobile phase, and the flow rate was 0.4 mL·min-1; The PDA detection wavelength was 190-400 nm; The column temperature was room temperature (30°C). RESULTS: The linear relationship between the concentration and peak areas of the six compounds were all linear (r>0.9996). The average recoveries (n=6) were between 99%-101%. CONCLUSION: This method was simple, accurate and reliable to determine the six contents of Zhikechuanbeipipa dropping pills for the quality control. Copyright 2012 by the Chinese Pharmaceutical Association.