Biotransformation of polydatin by rat intestinal bacteria / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the gastrointestinal metabolic transformation of polydatin, one of the main bioactive components in Rhizoma Polygoni, thus to figure out the possible biological modification exerted by rat intestinal bacteria in vitro and in vivo, and to determine the possible metabolites. METHODS: The biological samples were analyzed by high performance liquid chromatography (HPLC) and liquid chromatography mass spectrometer (LC-MS/MS) after treatments, respectively. RESULTS: Two main compounds, polydatin and resveratrol, were identified in rat gastrointestinal contents 4 h after the ig administration of polydatin 1500 mg·kg-1. CONCLUSION: This study revealed that polydatin could be metabolized to resveratrol by rat intestinal bacteria through desugarization. Copyright 2012 by the Chinese Pharmaceutical Association.