The killing effect of two different formulations paclitaxel in KB cell in vitro / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the killing effect of albumin-bound paclitaxel and paclitaxel solution on human oral epithelial carcinoma KB cells in vitro. METHODS: The KB cell line was cultured with different concentrations of albumin-bound paclitaxel or paclitaxel solution. MTT assay, inverted microscopic observation and flow cytometry assay were then used to monitor the growth and proliferation of the cell line. RESULTS: When treated with different concentrations of albumin-bound paclitaxel or paclitaxel solution for 36 and 72 h, KB cells survival rate decreased significantly, however, the difference between their IC50 was no significant; degeneration and necrosis of KB cells were found under microscope; the rate of cell apoptosis was increased gradually; the rate of S phase was reduced, and the percentage of G2/M cells treated with 0.1, 1 and 10 μmol · L-1 albumin-bound paclitaxel or paclitaxel was increased, which significantly higher than that of G2/M cells treated with 100 μmol · L-1 albumin-bound paclitaxel or paclitaxel. However, the two groups with the same concentration showed no significant differences in apoptosis rate and cell cycle (P > 0.05). CONCLUSION: Compared with paclitaxel, albumin-bound paclitaxel have the similar ability to inhibit proliferate and promote apoptosis in KB cells. Copyright 2012 by the Chinese Pharmaceutical Association.