Simultaneous determination of phenolic glycosides in Curculigins Rhizoma by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of phenolic glycosides in Curculigins Rhizoma. METHODS: Angilent 1100 HPLC on a ZORBAX SB C18 column(4.6 mm × 250 mm, 5 μm) with Extend C18 guard column(4.6 mm × 12.5 mm, 5 μm) were used. The mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid aqueous(B). The gradient elution program was as follows 0-25 min, 4%-6% A; 25-58 min, 6% - 17% A; 58-85 min, 17%-22% A The flow rate was kept at 1 mL · min-1, and the column temperature was set at 30°C. The detection wavelength was 230 nm. RESULTS: The concentrations and peak areas of the 8 phenolic glycosides including 5-hydroxymethylfurfural(1), 2-hydroxy-5-(2-hydroxyethyl) phenyl-β-D-glucopyranoside(2), anacardoside (3), orcinol glucoside (4), orcinol-1-O-β-D-apiofuranosyl-(1 → 6) -β-D-glucopyrano-side(5), 2, 6-dimethoxybenzoic acid(6), curculigoside(7) and curculigine A(8) were in good linear relationship. The average recoveries of the 8 constituents met the requirement for determination. CONCLUSION: The method is simple, sensitive, reproductive, and suitable for simultaneous determination of a variety of phenolic glycosides in Curculigins Rhizoma. Copyright 2012 by the Chinese Pharmaceutical Association.