Study on quality control of Ranunculi Ternati Radix / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 101-103, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-860841
ABSTRACT
OBJECTIVE:
To identify a characteristic chemical marker of Ranunculi Ternati Radix and establish a HPLC method for determination of its content.METHODS:
Various chromatographic methods were applied to isolate the marker 4-[formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl] butanoic acid, and its structure was elucidated by spectroscopic methods. The HPLC was performed on Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) using CH3OH-H2O (containing 0.4% acetic acid) as the mobile phase. The flow rate was 1.0 mL · min-1 and the detection wavelength was set at 292 nm.RESULTS:
4-[formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl] butanoic acid was isolated and identified as the chemical marker. The HPLC method showed good linearity within the range of 0.372-1.302 μg (r2=0.9998). The average recovery of 4-[formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl] butanoic acid was 100.89% (n=6, RSD=0.83%).CONCLUSION:
This method is simple, rapid and reliable for the quality control of Ranunculi Ternati Radix. Copyright 2012 by the Chinese Pharmaceutical Association.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2012
Tipo de documento:
Artigo
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