Effect of Nitric Oxide on the Viability of Bone Marrow - Derived Cultured Mast Cells / 대한면역학회지
Korean Journal of Immunology
;
: 595-600, 1997.
Artigo
em Coreano
| WPRIM
| ID: wpr-86126
ABSTRACT
It is well established that mast cell proliferation and maturation are regulated by two principle cytokines, IL-3 and the c-kit ligand stem cell factor (SCF). Previous reports have demonstrated that bone marrow-derived IL-3-dependent mast cells exhibit the characteristic apoptosis on removal of IL-3. To know how the number of mast cells is controlled, we observed the effects of nitric oxide (NO) on the murine bone marrow-derived cultured mast cells (BMCMC). Apoptosis was measured by the analysis of flow cytometric data and electrophoretic evidence of DNA fragmentation. Our data showed that sodiurn nitroprusside (SNP)-a NO releasing substance- induced apoptosis in BMCMC. Cell cycle analysis showed that the number of the G,/G, and S phase decreased markedly, while the percentage of cell in G,/M phase was increased. Also, SNP alone induced cell death, whereas SNP in combination with SCF markedly decreased cell death of BMCMC. SNP-induced apoptosis was partially inhibited by the treatment of BMCMC with SCF. Our results suggest that NO might have sorne role in the regulation of the number of mast cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Medula Óssea
/
Nitroprussiato
/
Ciclo Celular
/
Citocinas
/
Fase S
/
Interleucina-3
/
Morte Celular
/
Apoptose
/
Fator de Células-Tronco
/
Fragmentação do DNA
Idioma:
Coreano
Revista:
Korean Journal of Immunology
Ano de publicação:
1997
Tipo de documento:
Artigo
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