Mechanism of LncRNA DDX11-AS1 in Regulating Proliferation, Migration and Apoptosis of Gastric Cancer Cells by Targeting MiR-497-5p / 胃肠病学

ABSTRACT

Background: LncRNA expression was up-regulated or down-regulated in gastric cancer, but the mechanism of role of LncRNA in the development and progression of gastric cancer was not been fully clarified. Aims: To investigate the mechanism of LncRNA DDX11-AS1 in regulating the proliferation, migration and apoptosis of gastric cancer cells by targeting the expression of miR-497-5p. Methods: qRT-PCR was used to detect the expressions of DDX11-AS1 and miR-497-5p in gastric cancer tissue and cell lines. Pearson method was used to analyze the correlation between DDX11-AS1 and miR-497-5p in gastric cancer tissue. Gastric cancer HGC-27 cells were randomly divided into NC group, si-con group, si-DDX11-AS1 group, miR-con group, miR-497-5p group, si-DDX11-AS1+anti-miR-con group, si-DDX11-AS1+anti-miR-497-5p group. The cell proliferation was detected by MTT. The cell apoptosis was detected by flow cytometry. Transwell assay was used to detect the migration and invasion ability. The dual luciferase reporter system assay was used to verify the targeted regulatory relationship between DDX11-AS1 and miR-497-5p. The protein expressions of cyclin D1, cleaved caspase-3, MMP-2 and MMP-9 were detected by Western blotting. Results: The expression of DDX11-AS1 in gastric cancer tissue and cell lines was significantly increased (P<0.05), while the expression of miR-497-5p was significantly decreased (P<0.05), DDX11-AS1 was negatively correlated with miR-497-5p (r=-0.754, P<0.05). Interfering the expression of DDX11-AS1 or up-regulating the expression of miR-497-5p significantly inhibited cell proliferation, migration and invasion (P<0.05), apoptosis rate was significantly increased (P<0.05), and the expressions of cyclin D1, MMP-2 and MMP-9 were significantly decreased (P<0.05), and the expression of cleaved caspase-3 was significantly increased (P<0.05). The dual luciferase reporter assay demonstrated that DDX11-AS1 negatively regulated the expression and activity of miR-497-5p. Inhibition of miR-497-5p expression reversed the effect of interference with DDX11-AS1 expression on the biological behavior of gastric cancer cells. Conclusions: Interference with LncRNA DDX11-AS1 expression can inhibit the proliferation, migration, invasion and induce apoptosis of gastric cancer cells by targeting and up-regulating the expression of miR-497-5p.