Expressions and Significance of MMP-7 and SDC-1 in Inflammatory Bowel Disease / 胃肠病学

ABSTRACT

Background: Matrix metalloproteinase-7 (MMP-7), a member of the MMPs family, is expressed in injured mucosal epithelial cells of many organs. Syndecan-1 (SDC-1), a substrate of MMP-7, plays an important role in tissue repair and maintaining intestinal barrier function. Aims: To investigate the expressions and significance of MMP-7 and SDC-1 in inflammatory bowel disease (IBD). Methods: Forty-five intestinal mucosa samples and fifty peripheral blood samples from inpatients with active IBD were collected during Jan. 2017 to Jan.2018 at the Second Affiliated Hospital of Zhengzhou University. Thirty intestinal mucosa samples and thirty-five peripheral blood samples from non-IBD subjects were served as controls. Expressions of MMP-7 and SDC-1 in intestinal mucosal tissue were detected by immunohistochemistry, and serum SDC-1 level was determined by ELISA. Results: MMP-7 was highly expressed in intestinal mucosal tissue of IBD patients, especially in mucosal epithelium bordering the ulcer area. Compared with control group, the expression of MMP-7 was significantly increased and that of SDC-1 was significantly reduced in intestinal mucosal tissue of IBD patients (P<0.05). In contrast, the serum level of SDC-1 was significantly increased in IBD patients (P<0.05). Spearman correlation coefficient analysis revealed a positive correlation between MMP-7 expression and the disease activity of IBD (rs=0.519, P<0.05 for ulcerative colitis; rs =0.583, P<0.05 for Crohn's disease), and a negative correlation between MMP-7 and SDC-1 in inflamed mucosa (rs=-0.646, P<0.05). Conclusions: Increased MMP-7 expression in intestinal mucosal tissue may contribute to the initiation and development of IBD via cleaving SDC-1 and impairing mucosal healing.