Inhibitory Effect of Swertiamarin on MGC803 Cells in vitro and in vivo / 胃肠病学
Chinese Journal of Gastroenterology
;
(12): 86-89, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-861871
ABSTRACT
Background:
Gastric cancer is one of the most commonly seen malignant tumors. Inhibiting the proliferation of gastric cancer cells is the key for the development of anticancer drugs.Aims:
To investigate the effect of swertiamarin on proliferation of MGC803 cells in vitro and in vivo.Methods:
MGC803 cells were cultured with different concentrations of swertiamarin (0, 30, 60 and 90 μg/mL) for 48 hours. The cell activity were detected by MTT. The apoptosis rate was measured by Annexin V-FITC/PI assay. The contents of Bax and Bcl-2 were detected by ELISA. MGC803 cell xenograft tumor in BALB/c nude mice was established, and different concentrations of swertiamarin (0, 40, 60 and 80 mg/kg) were injected through caudal vein. Nude mice were killed when the volume of tumor was 1 000 mm3. The initial and final body weights of nude mice were recorded. The protein expression of Ki-67 was detected by immunohistochemistry.Results:
Compared with control group, different concentrations of swertiamarin effectively inhibited the proliferation of MGC803 cells, induced cell apoptosis, increased the content of Bax, while the content of Bcl-2 was significantly decreased (P<0.05). In BALB/c nude mice with MGC803 cell xenograft tumor, the volume of tumor, protein expression of Ki-67 decreased significantly in groups injected with different concentrations of swertiamarin than in control group (P<0.05).Conclusions:
Swertiamarin is a safe and effective anti-gastric tumor plant agent.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Gastroenterology
Ano de publicação:
2019
Tipo de documento:
Artigo
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