Expression of miR-1182 in ovarian cancer and its effect on tumor cell proliferation and metastasis / 国际生物医学工程杂志

ABSTRACT

Objective:To investigate the expression of miR-1182 in ovarian cancer tissues and its effect on tumor cell proliferation and metastasis.Methods:Reverse transcription polymerase chain amplification (RT-PCR) technology was used to detect the expression level of miR-1182 in normal ovarian tissue, ovarian cancer and corresponding adjacent tissues. The human ovarian cancer cell line SKOV-3 was transfected with miR-1182 mimics virus, and the expression levels of miR-1182 and hTERT protein were detected by RT-PCR and Western Blot respectively. The thiazolyl method was used to detect the proliferation and invasion of the transfected SKOV-3 cells.Results:The expression level of miR-1182 in ovarian cancer tissue was significantly lower than that in normal ovarian tissue and adjacent tissues (all P<0.05). Regarding the expression level of miR-1182, the difference between patients with different tumor stages and degree of tissue differentiation was statistically significant (all P<0.05), but the difference between patient age and tumor type was not statistically significant (all P>0.05). Compared with the blank virus group and the control group, the miR-1182 expression level of the transfected group was significantly increased (all P<0.05), while the expression of hTERT protein was significantly decreased (all P<0.05). The proliferation and migration ability of SKOV-3 cells in the transfected group was significantly inhibited at 24, 48, and 72 h after transfection (all P<0.05). Conclusions:The expression of miR-1182 is low in ovarian cancer tissues. Overexpression of miR-1182 can inhibit the proliferation and invasion of ovarian cancer, and the mechanism may be related to the targeted regulation of hTERT expression to achieve regulation.