GC-MS analysis of volatile oil chemical composition in the leaves of phlomis umbrosa from different habitats / 国际中医中药杂志

ABSTRACT

Objective:To explore the composition and content differences of volatile oil in the leaves of Phlomis umbrosa from different habitats, in order to clarify the chemical composition and content on efficacy.Methods:To collect the Phlomis umbrosa from Haoping area in Mei county and Sanyuan county of Shaanxi province. The volatile oil of Phlomis umbrosa was extracted by steam distillation method. Gas chromatography- mass spectrometry (GC-MS) was used to detect the chemical constituents of volatile oil.Results:Forty-nine kinds of volatile components were identified from the leaves collected in Haoping area of Mei county and fifty-six volatile component were identified from the leaves collected in Sanyuan county. There are five ingredients in common, excluding diacetone alcohol. 2-tert-butoxy-5-methylthiophene is the main volatile oil composition and the content reached 18.009% of leaves collected from Haoping area in Mei county, while paclitaxel is main volatile oil composition and the content reached 50.728% in Sanyuan county.Conclusions:The chemical compostion and content of volatile oil of Phlomis umbrosa in different habitats are different.