Study on the mechanism of Yishen-Qingli granule in treating IgA nephropathy based on network pharmacology / 国际中医中药杂志

ABSTRACT

Objective:To explore the molecular mechanism of Yishen-Qingli granules in treating IgA nephropathy through network pharmacology. Methods:The relevant components and functional targets of 8 Chinese herbs of Yishen-Qingli granules were screened through the TCMSP. The functional targets were converted into genes in the Uniprot database. Cytoscape 3.6.0 was used to construct the active ingredients-target network; screening IgA nephropathy-related targets through the Human Genome Database (GeneCards), importing drug and disease targets data into Venny2.1 software for intersection, and uploading the shared targets to the STRING platform to build a protein interaction network. With the help of bioinformatics resource database, GO and KEGG enrichment analysis were carried out on the generated results, and the multi-dimensional network of " Yishen-Qingli granule composition target signal pathway" was constructed. Results:69 active ingredients were screened, including ginsenoside, beta-sitosterol, sitosterol, and quercetin. There were 94 Yishen-Qingli granules for IgA nephropathy, including 30 core targets, including JUN, TNF, TP53, AKT1, and IL-6. KEGG enrichment analysis mainly involve three pathways, namely PI3K-Akt pathway, TNF inflammation pathway and MAPK pathway. Conclusions:Yishen-Qingli granules can treat IgA nephropathy in multi-component, multi-target and multi-pathway way. This study initially explored the therapeutic mechanism of Yishen-Qingli granules through network pharmacological analysis, and laid the foundation for further experimental research.