In vitro development of canine somatic cell nuclear transfer embryos in different culture media
Journal of Veterinary Science
;
: 233-235, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-86394
ABSTRACT
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Blastocisto
/
Clonagem de Organismos
/
Meios de Cultura
/
Técnicas de Cultura Embrionária
/
Desenvolvimento Embrionário
/
Cães
/
Técnicas de Transferência Nuclear
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2015
Tipo de documento:
Artigo
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