Effect of Brucea javanica oil emulsion on proliferation inhibition of multi-drug resistant K562/VCR cell line of human leukemia / 中国医师进修杂志
Chinese Journal of Postgraduates of Medicine
;
(36): 910-914, 2020.
Artigo
em Chinês
| WPRIM
| ID: wpr-865613
ABSTRACT
Objective:
To investigate the inhibitory effect of Brucea javanica oil emulsion on the proliferation of human leukemia multi-drug resistant K562/VCR cell line.Methods:
Methyl thiazolyl tetrazolim (MTT) colorimetric method was used to detect the proliferation inhibition rate of K562/VCR cells in vitro after intervention with Brucea javanica oil emulsion.Results:
With the increasing concentration of Brucea javanica oil emulsion (125,250,500,750,1 000 mg/L), the inhibition ratio to K562 cells continued to increase ( P<0.05), and with the increasing duration of Brucea javanica oil emulsion(24,48,72 h), the inhibition ratio to K562 cells continued to increase ( P<0.05). With the increasing concentration of Brucea javanica oil emulsion(500,750,1 000,1 500,2 000 mg/L), the inhibition ratio of K562/VCR cells continued to increase ( P<0.05), and with the increasing action time of Brucea javanica oil emulsion (24,48,72 h), the inhibition ratio of K562/VCR cells continued to increase ( P<0.05). With the increasing concentration of Brucea javanica oil emulsion(blank control group , Brucea javanica oil emulsion group for 500,750,1 000,1 500 mg/L), the G 0/G 1 ratio in K562/VCR cell cycle continued to increase, while the S ratio and G 2/M ratio continued to decrease ( P<0.05). After injection of Brucea javanica oil emulsion, ADM concentration (fluorescence intensity) in the cells increased significantly(161.4 ± 10.9 vs. 95.9 ± 8.1)( P<0.05).Conclusions:
Brucea javanica oil emulsion can inhibit the proliferation of multi-drug resistant K562/VCR cell lines and induce the arrest of multi-drug resistant cell cycle. Brucea javanica oil emulsion increasesd the accumulation of chemotherapeutic agents in multi-drug resistant K562/VCR cell lines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Postgraduates of Medicine
Ano de publicação:
2020
Tipo de documento:
Artigo
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