Effect of Clostridium perfringens combined with live bacteria therapy on intestinal flora, inflammation and immune indicators in children with pneumonia and antibiotic associated diarrhea / 中国基层医药

ABSTRACT

Objective:To investigate the effect of Clostridium perfringens combined with live bacteria therapy on intestinal flora, inflammation and immune indicators in children with pneumonia and antibiotic associated diarrhea.Methods:From December 2016 to October 2019, 44 neonates with pneumonia and antibiotic associated diarrhea diagnosed and treated in the Sixth People's Hospital of Datong were selected in the study, and they were divided into control group and observation group according to the random number table method, with 22 cases in each group.On the basis of routine symptomatic treatment, the control group was treated by oral administration of Saccharomyces boulardii, and the observation group was treated with Clostridium perfringens combined with live bacteria powder.The treatment course was two weeks.The intestinal flora, inflammatory factors and immune function of the two groups were compared.Results:There were no statistically significant differences in the levels of IL-2, IL-6 and TNF-α between the two groups before treatment(all P>0.05), and the levels of IL-2, IL-6 and TNF-α in the two groups after treatment were significantly improved(all P<0.05). The levels of IL-2[(34.20±8.55)ng/L vs.(40.13±10.32)ng/L], IL-6[(21.02±5.66)ng/L vs.(31.25±7.82)ng/L] and TNF-α[(30.10±6.20)ng/L vs.(43.55±9.52)ng/L] in the observation group were significantly lower than those in the control group( t=7.501, 6.885, 8.264, all P<0.05). There were no statistically significant differences in the number of Lactobacillus, Bifidobacterium and Eubacterium in the two groups before treatmentt(all P>0.05), and the number of Lactobacillus, Bifidobacterium and Eubacterium in the two groups after treatment increased significantly(all P<0.05). The number of Lactobacillus[(9.50±0.53)×10 8 CFU/mL vs.(8.95±0.44)×10 8 CFU/mL], Bifidobacterium[(10.60±0.53)×10 8 CFU/mL vs.(9.90±0.46)×10 8 CFU/mL] and Eubacterium[(8.93±0.68)×10 8 CFU/mL vs.(8.08±0.40)×10 8 CFU/mL] in the observation group were significantly higher than those in the control group( t=5.065, 6.547, 5.116, all P<0.05). There were no statistically significant differences in the levels of CD 3+ , CD 4+ , CD 8+ and CD 4+ /CD 8+ between the two groups before treatment(all P>0.05), and the levels of CD 3+ , CD 4+ , CD 8+ and CD 4+ /CD 8+ in the two groups after treatment were significantly improved(all P<0.05). The levels of CD 3+ [(62.55±5.06)% vs.(57.12±4.39)%], CD 4+ [(40.52±3.52)% vs.(36.43±2.55)%], CD 4+ /CD 8+ [(1.78±0.22) vs.(1.43±0.13)] in the observation group were significantly higher than those in the control group( t=7.504, 6.958, 5.452, all P<0.05). The level of CD 8+ [(21.22±2.05)% vs.(25.44±2.49)%] in the observation group was significantly lower than that in the control group( t=5.112, P<0.05). Conclusion:Clostridium perfringens combined with live bacteria in the treatment of neonatal pneumonia with antibiotic associated diarrhea can improve intestinal flora, reduce inflammatory response and improve immune function, and the effect is better.