Effects of sodium selenite on motor function and antioxidant capacity of substantia nigra in rats with Parkinson's disease / 中华行为医学与脑科学杂志

ABSTRACT

Objective:To investigate the effects of sodium selenite on the motor function and antioxidant capacity of substantia nigra in Parkinson's disease (PD) rats.Methods:A total of 48 male SD rats with 0 score in pole test were selected, 8 of which were used as control group (group I) and the remaining 40 rats were intraperitoneally injected with MPTP for 7 days to establish the PD model.The 24 PD rats with successful modeling were selected out by behavioral analysis, and randomly divided into MPTP group (group Ⅱ), MPTP+ 0.05 mg/kg Se group (group Ⅲ), MPTP+ 0.1 mg/kg Se group (group Ⅳ), with 8 rats in each group.After 30 days of gavage, the behaviors of lipid peroxides in the substantia nigra, the activities of glutathione peroxidase and superoxide dismutase, pathological changes in the substantia nigra.The number of TH + cells and TH mRNA levels in each group were analyzed. Results:Compared with group Ⅰ, the number of crossing of group II was significantly decreased((95.40±14.66), (6.11±4.17), P<0.05), and rearing was significantly decreased and the score of pole test was significantly increased ( P<0.05). MDA content was significantly increased((4.02±0.62), (12.75±1.59), P<0.05). The activity of SOD and GSH-Px decreased significantly( P<0.05). The number of neurons in nigra decreased, the number of TH + cells and the expression level of TH mRNA decreased significantly( P<0.05). Compared with group II, group III and group IV showed a significant increase in open field test((88.80±24.61), (38.86±19.77), P<0.05), and a significant decrease in pole test scores ( P<0.05). MDA content in group Ⅲ was significantly reduced ( P<0.05). SOD and GSH-Px activity increased significantly ( P<0.05). The nerve cells in the substantia nigra region had complete structure and orderly arrangement, and the number of TH + cells and the expression level of TH mRNA were significantly increased ( P<0.05). There was no difference in MDA content in group.SOD and GSH-Px activity increased, but the difference was not significant.The number of TH + cells increased, but the difference was not significant. Conclusion:It suggests that 0.05 mg/kg of sodium selenite can significantly improve the motor function of PD rats and enhance the antioxidant capacity of substantia nigra, so as to protect neurons in the substantia nigra.