Upregulation of IP-10(CXCL10) mRNA Expression by Interleukin-18 / 영남의대학술지
Yeungnam University Journal of Medicine
;
: 67-78, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-8718
ABSTRACT
BACKGROUND:
Interleukin-18 (IL-18) is one of the principal inducers of interferon-gamma (IFN-gamma) in lymphocytes. MATERIALS ANDMETHODS:
The effect of IL-18 on the expression of chemokine IP-10(CXCL10) mRNA in C57BL/6 mouse peritoneal macrophages was studied by using Northern blot analysis, enzyme linked immunosobent assay and electrophoretic mobility shift assay.RESULTS:
IL-18 was determined to exert no direct effect on the expression of IP-10(CXCL10) mRNA. However, IL-18 pretreatment was determined to play a cooperative role in the synergistic induction of LPS-induced IP-10(CXCL10) mRNA expression. The effect associated with IL-18 pretreatment with regard to the synergistic induction of LPS-induced IP-10 (CXCL10) mRNA expression was detected after 16 hr of IL-18 pretreatment, administered prior to LPS stimulation. The pattern of NF-kB binding activity during IL-18 pretreatment with LPS stimulation was found to coincide with the expression of IP-10(CXCL10) mRNA.CONCLUSION:
Although IL-18 alone exerts no direct effect on the expression of chemokine IP-10(CXCL10), a definite period of IL-18 pretreatment induces the synergistic expression of LPS-induced IP-10(CXCL10) mRNA. NF-kB activation is a component of this synergistic effect of IL-18 pretreatment. These results provide useful information, which may facilitate the elucidation of the action mechanisms underlying IL-18 effect on the expression of IP-10(CXCL10) mRNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Linfócitos
/
Regulação para Cima
/
Northern Blotting
/
NF-kappa B
/
Interferon gama
/
Macrófagos Peritoneais
/
Interleucina-18
/
Ensaio de Desvio de Mobilidade Eletroforética
Limite:
Animais
Idioma:
Inglês
Revista:
Yeungnam University Journal of Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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