GC-MS Analysis of Volatile Oil of Notopterygium inchum and Notopterygium franchetii / 中国实验方剂学杂志

ABSTRACT

Objective:To study the volatile oil of Notopterygium inchum and N. franchetii by using gas chromatography-mass spectrometry （GC-MS）. Method:Ten batches of N. inchum and N. franchetii were harvested from Sichuan and Gansu，including eight batches of wild samples， one batch of cultivated N. inchum and one batch of cultivated N. franchetii. Volatile oil was extracted according to the methods in Chinese Pharmacopeia， and the chemical constituents were analyzed with GC-MS technology. Result:Ninety-seven chemical compounds were identified，including monoterpenes，sesquiterpenes and non-terpenes. The calculation results of the relative content of chemical components showed that α-pinene（25.39%-41.68%），β-pinene（19.34%-40.95%） and limonene（4.59%-11.99%） were main compounds in volatile oil of N. inchum and N. franchetii. Relative content of α-pinene was higher than that of β-pinene in wild N. inchum and cultivated N. franchetii， while relative content of β-pinene was higher than that of α-pinene in wild N. franchetii. Relative content of β-pinene in samples stored for one year was lower than that of newly harvested ones. The partial least squares discriminant analysis with SIMCA-P+11.5 software showed that the samples from Gansu and Sichuan provinces had no significant differences， and additionally，one-year-stored samples and newly-harvested samples were clustered into two groups. Conclusion:This study showed that the storage had influence on the chemical constituents of volatile oil in N. inchum， and it also revealed the differences in volatile oil components of wild and cultivated Notopterygium. The above mentioned work provided chemical basis for the pharmacology comparison study of volatile oil from different kinds of N. inchum and N. franchetii.