Effect of Different Arbuscular Mycorrhizal Fungi Combinations and Inoculation Periods on Rhizosphere Soil Physicochemical Properties and Microbial Quantity of Paris polyphylla var. yunnanensis Seedlings / 中国实验方剂学杂志

ABSTRACT

Objective:To study the effects of different arbuscular mycorrhizal （AM） fungi combinations on rhizosphere soil physicochemical properties and microbial quantity in the seedlings of Paris polyphylla var. yunnanensis at different inoculation periods，so as to lay a foundation for cultivating high-quality P. polyphylla var. yunnanensis. Method:The spore density，infection rate，nutrient and enzyme activity in the soil around the roots of P. polyphylla var. yunnanensis seedlings under different AM fungi combinations and different inoculation periods were analyzed by the greenhouse pot inoculation trials and soil agrochemical analysis methods. Result:The infection rate of different AM fungi treatment groups was more than 80% in different inoculation periods，and the spore density was higher than control （CK） group in some periods. It reflected that the relationship between AM fungi and roots of Paris polyphylla seedlings was favorable. The content of nitrogen in the soil decreased，but the content of available P，available K and soil pH increased. The soil nutrients in the cultivar one-year seedlings and wild seedlings were higher. The total number of soil microorganisms showed that bacteria>actinomycetes>fungi. The cultivar two-year seedlings with AM fungi combinations of S3，S5 and S8 had better soil structure and higher biomass carbon content. The growth rates of phosphatase and protease activity were higher in the soil，but catalase activity was lowest. In the treatment groups S2，S3，S4，S5 and S6，the soil enzyme activities of the wild seedlings and one-year-old seedlings were best. Conclusion:Different AM fungal treatment groups and different inoculation periods had certain effects on rhizosphere soil physicochemical properties and microbial quantity in the seedlings of P. polyphylla var. yunnanensis， which provided a technical basis for the cultivation of P. polyphylla var. yunnanensis.