Identification of Multi-source Ethnodrug Rodgersiae Rhizoma by DNA Barcoding Technology / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 142-150, 2020.
Article
em Zh
| WPRIM
| ID: wpr-872932
Biblioteca responsável:
WPRO
ABSTRACT
Objective:Five popular DNA barcoding sequences,namely ITS,ITS2,rbcL,matK and psbA-trnH,were employed to evaluate the identification efficiency of multi-source ethnodrug Rodgersiae Rhizoma,and the most suitable sequence was then screened out. Method:Efficiency of polymerase chain reaction(PCR) amplification,success rate of sequencing,intra- and inter-specific distances calculated by rank sum test,phylogenetic tree constructed with neighbor-joining(NJ) method and identification efficiency assessed by Blast 1 and NJ method were adopted in this study. Result:Efficiency of PCR amplification and success rate of sequencing for ITS,ITS2,psbA-trnH,rbcL and matK were 100%,96.61% ,100%,98.31%,100%,100%,100%,100%,98.31% and 98.31%,respectively. Intra- and inter-specific genetic distances and identification achievement rate for psbA-trnH were the highest among the five candidate sequences. Besides,the average coalescent depth was less than the smallest interspecific distance for psbA-trnH. Phylogenetic tree also illustrated that Rodgersiae Rhizoma could be distinguished based on psbA-trnH. Conclusion:According to the findings,psbA-trnH was superior to other DNA barcodes. Therefore, psbA-trnH was recommended as the ideal DNA barcode for the identification of multi-source ethnodrug Rodgersiae Rhizoma.
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Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Experimental Traditional Medical Formulae
Ano de publicação:
2020
Tipo de documento:
Article