Molecular Analysis of Oculocutaneous Albinism Patients in Korea
Annals of Dermatology
;
: 182-187, 1997.
Artigo
em Inglês
| WPRIM
| ID: wpr-87320
ABSTRACT
BACKGROUND:
Oculocutaneous albinism (OCA) is a genetic disorder of the melanin pigment system in which melanin synthesis is reduced or absent in the skin, hair, and eyes. OCA is classified into two major types, and tyrosinase-related OCA can be produced by mutations of the structural gene for tyrosinase enzyme (TYR gene).OBJECTIVE:
The purpose of this study was to analyze the segregation of mutant alleles of the TYR gene in tyrosinase-negative and tyrosinase-positive Korean OCA patients and families.METHODS:
We amplified exon I, II, and III of the TYR gene of Korean OCA patients and their families by polymerase chain reactions (PCR), and analyzed the mutations by restriction fragment length polymorphism (RFLP) analysis in exon I and single-strand conformation polymorphism (SSCP) analyses in exon II and exon III.RESULTS:
Two tyrosinase-negative cases showed mutations in exon I. Four tyrosinase-nega-tive cases and one tyrosinase-positive case showed mutations in exon II, and one tyrosinase-neg- ative case showed mutations in exon III. In summary, we found three kinds of mutation in four tyrosinase-negative OCA patients and one tyrsinase-positive OCA patient.CONCLUSIONS:
RFLP and SSCP analysis can provide a basis for a rapid and sensitive screening system to detect TYR gene mutations of Korean OCA patients and their families.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pele
/
Polimorfismo de Fragmento de Restrição
/
Programas de Rastreamento
/
Reação em Cadeia da Polimerase
/
Éxons
/
Albinismo Oculocutâneo
/
Monofenol Mono-Oxigenase
/
Polimorfismo Conformacional de Fita Simples
/
Alelos
/
Cabelo
Tipo de estudo:
Estudo de rastreamento
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
1997
Tipo de documento:
Artigo
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