Effect of over-expression of vasohibin-1 on malignant biological behavior of human colorectal cancer cells / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6): 121-127, 2021.
Artigo
em Chinês
| WPRIM
| ID: wpr-875833
ABSTRACT
@#[Abstract] Objective:
To investigate the effects of over-expressing VASH1 on the malignant biological behaviors of human colorectal cancer cells.Methods:
Lentivirus was packaged and transfected into human colorectal cancer cells SW680 and SW620 to construct an over-expressed VASH1 cell line, the untransfected cells were used as control. qPCR experiment and WB experiment were used to detect the over-expression effect of VASH1. The effects of VASH1 over-expressed on microangiogenesis, proliferation, colony formation and migration of colorectal cancer cells were detected respectively by tubule formation, CCK-8 assay, soft agar assay, Transwell assay and Wound healing assay in vitro. In addition, tumor growth and lung metastasis were detected in NOD-SCID mice subcutaneously injected with VASH1-overexpressing SW620 cells.Results:
Successfully constructed SW480 and SW620 cells over-expressing VASH1. Compared with the control group, the abilities of microangiogenesis, proliferation, colony forming and migration were significantly reduced in colorectal cancer cells over-expressing VASH1 (P<0.05) in vitro. The abilities of subcutaneous tumor growth and lung metastasis of colorectal cancer cells over-expressing VASH1 were also significantly reduced (P<0.05) in vivo.Conclusion:
Over-expression of VASH1 can suppress the malignant biological behaviors of human colorectal cancer cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Cancer Biotherapy
Ano de publicação:
2021
Tipo de documento:
Artigo
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