Research on the oxidation damage of sodium arsenite on rat lung / 中国职业医学

ABSTRACT

OBJECTIVE: To explore the oxidation damage of sodium arsenite( NaAsO_2) drinking exposure on rat lung.METHODS: Thirty-two specific pathogen free healthy adult male SD rats were randomly divided into 4 groups control group,low-,medium- and high-dose groups,with 8 rats in each group. The 3 arsenic exposure groups were intoxicated by NaAsO_2 with mass concentrations of 10. 00,100. 00 and 1 000. 00 μg / L in drinking water,respectively,while the control group was given ultrapure water for free drinking. All the rats were sacrificed after 28 days. The total number of white blood cells and the cell death rate in bronchoalveolar lavage fluid( BALF) were measured. The level of reactive oxygen species( ROS),nicotinamide adenine dinucleotide phosphate( NADPH),the antioxidant ability and the nitric oxide( NO) level in BALF were measured by the enzyme-linked immunosorbent assay. RESULTS: The total number of white blood cells in BALF in medium-dose group was higher than those of the control group and high-dose group( P < 0. 05).The cell death rate of BALF in high-dose group was higher than those of the other groups( P < 0. 01). The ROS levels in BALF of rat lung in 3 exposure groups were higher than that of the control group( P < 0. 01). The NADPH levels in medium- and high-dose groups were higher than those of the control group and low-dose group( P < 0. 05). The total antioxidant ability in medium- and high-dose groups were lower than that of the control group( P < 0. 01). The NO levels in medium- and high-dose groups were higher than that of the control group( P < 0. 05). CONCLUSION: The NaAsO_2 exposure in rats could lead to high expression of oxidase ROS by activating NADPH followed by increasing NO level,resulting in imbalanced organism oxidation and anti-oxidation system and causing oxidative stress injury in tissues.