RT-nPCR Assays for Amplification and Sequencing of VP1 Genes in Human Enterovirus A-D from Clinical Specimens / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 829-838, 2020.
Article
em En
| WPRIM
| ID: wpr-878347
Biblioteca responsável:
WPRO
ABSTRACT
Objective@#To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses (HEVs) from clinical samples and to contribute to etiological surveillance of HEV-related diseases.@*Methods@#A panel of RT-nPCR assays, consisting of published combined primer pairs for VP1 genes of HEV A-C and in-house designed primers for HEV-D, was established in this study. The sensitivity of each RT-nPCR assay was evaluated with serially diluted virus stocks of five serotypes expressed as CCID @*Results@#The sensitivity of RT-nPCR assays for amplifying partial VP1 gene of HEVs was 0.1 CCID @*Conclusion@#This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of HEV A-D, providing rapid, sensitive, and reliable options for molecular typing and molecular epidemiology of HEVs in clinical specimens.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Epidemiologia Molecular
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Enterovirus Humano B
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Enterovirus Humano A
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Enterovirus Humano C
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Enterovirus Humano D
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Proteínas do Capsídeo
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Tipagem Molecular
Limite:
Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2020
Tipo de documento:
Article