A new quantitative 16S rRNA amplicon sequencing method / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2548-2555, 2020.
Artigo
em Chinês
| WPRIM
| ID: wpr-878510
ABSTRACT
In recent years, 16S rRNA amplicon sequencing technology has been widely used to study human gut microbiota and to detect unknown pathogens in clinical samples. However, its resolution to bacterial population can only reach the relative abundance of genus level, and different factors affect the final bacterial profile, such as sample concentrations, PCR cycle numbers and amplification primers. In order to solve these problems, we developed a quantitative 16S rRNA amplicon sequencing method by combining random tag and internal marker method. The new methods improved the accuracy of human gut microbiota, reduced the impact of experimental operation on the results, and improved the comparability between sequencing and other molecular biological methods.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bactérias
/
RNA Ribossômico 16S
/
Reação em Cadeia da Polimerase
/
Sequenciamento de Nucleotídeos em Larga Escala
/
Microbioma Gastrointestinal
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2020
Tipo de documento:
Artigo
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