Effect of autophagy on oxalate-induced toxicity of human proximal renal tubular epithelial cell / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 221-226, 2021.
Artigo
em Inglês
| WPRIM
| ID: wpr-880648
ABSTRACT
OBJECTIVES@#To investigate the role of autophagy in oxalate-induced toxicity of human proximal renal tubular epithelial cell (HK-2).@*METHODS@#HK-2 cells were exposed to oxalate (1 mmol/L) for 2 h and 3-methyladenine (3-MA) was used to inhibit autophagy. Then Western blotting was used to measure the expression of autophagy-related protein LC3II. Cell viability and cell apoptosis were measured by MTT assay and flow cytometry assay, respectively.@*RESULTS@#Cytoplasmic vacuolization was observed in HK-2 cells after treating with oxalate for 2 h. However, 3-MA showed no effects on the formation of cytoplasmic vacuolization regardless of the dose at 1 or 5 mmol/L. The expression of LC3II protein was significantly increased in the HK-2 cells in the presence of oxalate (0.62±0.03 vs 0.35±0.02, @*CONCLUSIONS@#Autophagy of HK-2 cells is enhanced by oxalate at the concentration of 1 mmol/L. Inhibition of 3-MA-induced autophagy protects HK-2 cells from the oxalate-induced cytotoxicity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxalatos
/
Autofagia
/
Linhagem Celular
/
Apoptose
/
Células Epiteliais
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2021
Tipo de documento:
Artigo
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