Silencing long non-coding RNA HIF1A-AS2 inhibits proliferation, invasion and migration of cervical cancer cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1615-1621, 2020.
Artigo
em Chinês
| WPRIM
| ID: wpr-880799
ABSTRACT
OBJECTIVE@#To explore the inhibitory effects of silencing long non-coding RNA (LncRNA) HIF1A-AS2 on epithelialmesenchymal transition (EMT) and tumor stem cell-like phenotype in cervical cancer cells.@*METHODS@#We designed 3 shRNA constructs for silencing HIF1A-AS2 in CaSki cells, and the shRNA with the strongest interference effect was selected for subsequent experiment. CaSki cells were transfected with shRNA-NC or Sh-HIF1A-AS2, and the changes in cell viability, invasion ability, EMT, expressions of EMT-related proteins, formation of cell spheres and expressions of stem cell markers were detected.@*RESULTS@#Transfection with shRNA-NC and Sh-HIF1A-AS2 did not significantly affected the viability of CaSki cells (@*CONCLUSIONS@#Silencing HIF1A-AS2 can inhibit proliferation, invasion and migration of cervical cancer cells
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Regulação Neoplásica da Expressão Gênica
/
Movimento Celular
/
Neoplasias do Colo do Útero
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
Transição Epitelial-Mesenquimal
/
RNA Longo não Codificante
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2020
Tipo de documento:
Artigo
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