Correlation between hearing-related gene polymorphism and susceptibility of noise-induced hearing loss / 中国职业医学

ABSTRACT

OBJECTIVE: To examine the correlation between single nucleotide polymorphism( SNP) in 3' untranslated regions and hearing-related genes and their correlation with susceptibility in noise-induced hearing loss( NIHL) in Chinese Han population. METHODS: A total of 2 507 workers exposed to 72-120 d B( A) of normalized continuous A-weighted sound pressure level equivalent to a 40 h-working-week intensity of continuous noise in three large compressor manufacturing enterprises in Guangzhou were chosen as study subjects by judgment sampling method. A model was set up to define sensitive group( 238 sensitive workers) and resistant group( 238 resistant workers) by testing the workplace noise intensity and worker hearing pure tone threshold test. The genomic DNA from peripheral blood was collected from workers of these two groups. The genetic characteristic analysis was carried out by using the Taq Man probe with chemical fluorescence allelic identification test. RESULTS: The monaural threshold of weighted value( MTWV) of the left ear in sensitive group was higher than that of the right ear( P < 0. 01); the MTWV of left ear and right ear in sensitive group were respectively higher than that of the same ear in resistant group( P < 0. 01). A total of four candidate genes were screened vesicle associated membrane protein 1( VAMP1),fibroblast growth factor 1( FGF1),potassium inwardly-rectifying channel,subfamily J,member 10( KCNJ10) and myosin IC( MYO1C). The results of SNP loci detection showed that more workers in sensitive group carried FGF1 rs17217562 AC and CC genotype than that of resistant group( P < 0. 05). More workers in sensitive group carried C allele of FGF1 rs17217562 than the resistant group( P < 0. 05). The logistic regression analysis showed that after correcting the confounding factors including age,noise exposure level,length of noise exposure,gender,smoking,drinking,whether or not using headset,organic solvents exposure,heavy metal exposure,high temperature exposure and hand-arm vibration exposure,the people carrying allele of FGF1 rs17217562 had an increased risk of NIHL susceptibility( P < 0. 05). The VAMP1,KCNJ10 and MYO1C gene had no susceptibility correlation with SNP and NIHL.CONCLUSION: Among Chinese Han population,SNP loci located on the FGF1 rs17217562 may be correlated with the susceptibility of NIHL.