Expression of Survivin and HIAP-1 in Korean Gastric Cancers
Journal of the Korean Gastric Cancer Association
;
: 19-25, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-88533
ABSTRACT
PURPOSE:
Dysregulation of apoptosis may attribute to development of cancer by abnormally prolonging cell viability with accumulation of transforming mutations. Survivin and HIAP (Human Inhibitors of Apoptosis)-1 were recently described as apoptosis inhibitors. Their pathogenic roles in gastric cancer are largely unknown. In the present study, we examined the expression of survivin and HIAP-1 in gastric cancer tissues and cell lines in order to elucidate the roles of survivin and HIAP-1 in the process of gastric carcinogenesis. MATENRIALS ANDMETHODS:
Eight gastric cancer cell lines and five gastric cancer tissues were studied. The expression of survivin and HIAP-1 were evaluated by reverse transcription -polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot.RESULTS:
Western blot and RT-PCR analysis revealed survivin and HIAP-1 expression in all gastric cancer cell lines. Increased expression of survivin and HIAP-1 were found in all cases of gastric cancer tissues compared to normal tissues by Western blot analysis. In immunohistochemical analysis tumor cells were stained with anti-survivin and anti-HIAP-1 antibodies. Cell cycle dependence of survivin expression was preserved in gastric cancer cell lines.CONCLUSION:
The results indicate that increased expression of survivin and HIAP-1 genes may play an important role in gastric cancer.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Gástricas
/
Imuno-Histoquímica
/
Ciclo Celular
/
Linhagem Celular
/
Sobrevivência Celular
/
Western Blotting
/
Apoptose
/
Transcrição Reversa
/
Carcinogênese
/
Anticorpos
Idioma:
Coreano
Revista:
Journal of the Korean Gastric Cancer Association
Ano de publicação:
2003
Tipo de documento:
Artigo
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