Your browser doesn't support javascript.
loading
Expression of Survivin and HIAP-1 in Korean Gastric Cancers
Journal of the Korean Gastric Cancer Association ; : 19-25, 2003.
Artigo em Coreano | WPRIM | ID: wpr-88533
ABSTRACT

PURPOSE:

Dysregulation of apoptosis may attribute to development of cancer by abnormally prolonging cell viability with accumulation of transforming mutations. Survivin and HIAP (Human Inhibitors of Apoptosis)-1 were recently described as apoptosis inhibitors. Their pathogenic roles in gastric cancer are largely unknown. In the present study, we examined the expression of survivin and HIAP-1 in gastric cancer tissues and cell lines in order to elucidate the roles of survivin and HIAP-1 in the process of gastric carcinogenesis. MATENRIALS AND

METHODS:

Eight gastric cancer cell lines and five gastric cancer tissues were studied. The expression of survivin and HIAP-1 were evaluated by reverse transcription -polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot.

RESULTS:

Western blot and RT-PCR analysis revealed survivin and HIAP-1 expression in all gastric cancer cell lines. Increased expression of survivin and HIAP-1 were found in all cases of gastric cancer tissues compared to normal tissues by Western blot analysis. In immunohistochemical analysis tumor cells were stained with anti-survivin and anti-HIAP-1 antibodies. Cell cycle dependence of survivin expression was preserved in gastric cancer cell lines.

CONCLUSION:

The results indicate that increased expression of survivin and HIAP-1 genes may play an important role in gastric cancer.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Neoplasias Gástricas / Imuno-Histoquímica / Ciclo Celular / Linhagem Celular / Sobrevivência Celular / Western Blotting / Apoptose / Transcrição Reversa / Carcinogênese / Anticorpos Idioma: Coreano Revista: Journal of the Korean Gastric Cancer Association Ano de publicação: 2003 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Neoplasias Gástricas / Imuno-Histoquímica / Ciclo Celular / Linhagem Celular / Sobrevivência Celular / Western Blotting / Apoptose / Transcrição Reversa / Carcinogênese / Anticorpos Idioma: Coreano Revista: Journal of the Korean Gastric Cancer Association Ano de publicação: 2003 Tipo de documento: Artigo