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Study on the relationship between the protective effect of 17β-estradiol on human lens epithelial cells and cell pyroptosis / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science ; (12): 1680-1685, 2021.
Artigo em Chinês | WPRIM | ID: wpr-886703
ABSTRACT
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AIM:

To explore the relationship between the protective effect of 17β-estradiol(E<sub>2</sub>)on human lens epithelial cells and pyroptosis. <p>

METHODS:

Human lens epithelial cells were cultured <i>in vitro</i> and divided into blank control group, H<sub>2</sub>O<sub>2</sub> treatment group, and 17β-estradiol+H<sub>2</sub>O<sub>2</sub> treatment group. Scanning electron microscope to observe the cytological morphology; immunofluorescence technique to detect Gasdermin D(GSDMD)distribution and fluorescence intensity; CCK-8 to detect cell viability; TUNEL to detect cell pyroptosis; Western-blot to detect Cysteinylaspartate specific proteinase-1(Caspase-1), GSDMD, NOD-like receptor protein 3(NLRP3)protein expression level; ELISA to detect interleukin-1β(IL-1β)expression. <p>

RESULTS:

Compared with the control group, the cell viability of the H<sub>2</sub>O<sub>2</sub> treatment group was significantly decreased, the expression of Caspase-1, GSDMD, and NLRP3 protein were significantly up-regulated, and the secretion of IL-1β was significantly increased. Compared with the H<sub>2</sub>O<sub>2</sub> treatment group, the expression of Caspase-1, GSDMD, and NLRP3 protein in the 17β-estradiol+H<sub>2</sub>O<sub>2</sub> treatment group were down-regulated, and the secretion of IL-1β decreased, and it showed a decreasing trend with the increase of estrogen concentration. <p>

CONCLUSION:

17β-estradiol has a protective effect on human lens epithelial cells, and its protective mechanism is related to the inhibition of the pyroptosis process of human lens epithelial cells, and the classical pyroptosis pathway is involved.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: International Eye Science Ano de publicação: 2021 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: International Eye Science Ano de publicação: 2021 Tipo de documento: Artigo