Effect and Mechanism of Sulforaphane on G / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1050-1055, 2021.
Artigo
em Chinês
| WPRIM
| ID: wpr-888517
ABSTRACT
OBJECTIVE@#To investigate the effect of sulforaphane (SFN) on G@*METHODS@#KG1a and KG1cells were treated by different concentrations of SFN for 48 h. Flow cytometry (FCM) was used to analyze the phase distribution of cell cycle. High-throughput sequencing was used to detect the effect of SFN on the expression of cell cycle related genes in KG1a cells. The mRNA expression of P53, P21, CDC2 and CyclinB1 were detected by qPCR. The protein expression of P53, CDC2, P-CDC2 and CyclinB1 were detected by Western blot.@*RESULTS@#Cells in the G@*CONCLUSION@#SFN induces leukemia cells to block in G
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sulfóxidos
/
Leucemia Mieloide Aguda
/
Ciclo Celular
/
Isotiocianatos
/
Mitose
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2021
Tipo de documento:
Artigo
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