Expression of Platelet Derived Growth Factor-A, C and Platelet Derived Growth Factor Receptor-alpha in the Ischemia Reperfusion Renal Failure Model / 대한신장학회잡지
Korean Journal of Nephrology
; : 13-22, 2006.
Article
em Ko
| WPRIM
| ID: wpr-89288
Biblioteca responsável:
WPRO
ABSTRACT
BACKGOUND: Platelet-derived growth factor (PDGF) is a widely expressed growth factor with both mitogenic and chemotactic activities in many connective tissue cell types. There are four members of PDGF family; PDGF-A, PDGF-B, PDGF-C, PDGF-D. Their biological effects are mediated via two tyrosine kinase receptors, PDGF receptor-alpha and PDGF receptor-beta, and PDGF-mediated signaling is critical for development of many organ systems and acquired disease. The aims of this study were to determine the changes of PDGF-A, PDGF-C and PDGF receptor (PDGFR)-alpha expression in ischemia reperfusion acute renal failure model. METHODS: We examined the expression and localization of PDGF-A, PDGF-C and PDGF receptor-alpha protein using Western blot analysis and immunohistochemistry and PDGF-C mRNA using RNase protection assay after ischemia reperfusion renal failure model. RESULTS: PDGF-A expression showed no change after ischemia reperfusion injury. Proliferating cell nuclear antigen expression increased at day 2 after ischemia reperfusion injury. PDGF-C expression increased at day 2 after ischemia reperfusion injury, and was localized in tubular epithelial cells of outer medulla. PDGFR-alpha increased at day 2 after ischemia reperfusion injury, and was localized in tubular interstitium of outer medulla. CONCLUSION: These results indicated that PDGF-C and PDGF receptor-alpha may have an important role in the renal regeneration after ischemia reperfusion renal injury.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Regeneração
/
Ribonucleases
/
Plaquetas
/
Fator de Crescimento Derivado de Plaquetas
/
RNA Mensageiro
/
Imuno-Histoquímica
/
Reperfusão
/
Traumatismo por Reperfusão
/
Western Blotting
/
Receptores do Fator de Crescimento Derivado de Plaquetas
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Nephrology
Ano de publicação:
2006
Tipo de documento:
Article