Blockade of Nitric Oxide Synthesis Further Diminishes Aquaporin Water Channels in Rat Kidney Subjected to Ischemia/reperfusion Injury / 대한신장학회잡지
Korean Journal of Nephrology
;
: 7-12, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-89289
ABSTRACT
BACKGOUND The present study examined whether a blockade of nitric oxide (NO) synthesis affects the regulation of aquaporin (AQP) water channels in rats subjected to renal ischemia/reperfusion (I/R). METHODS:
Renal I/R was experimentally induced by clamping the left renal artery for 60 minutes in rats. The rats were kept for 7 days thereafter, during which they were supplied with tap water containing NG-nitro-L-arginine methyl ester (L-NAME, 100 mg/L). The expression of AQP1-3 was determined in the kidney by Western blot analysis.RESULTS:
In renal I/R injury, the expression of AQP2 was significantly decreased. The treatment with L-NAME further diminished the expression of AQP2. Although the expression of either AQP1 or AQP3 was not significantly altered in the kidney subjected to I/R, it was also significantly decreased by the treatment with L-NAME.CONCLUSION:
It is suggested that endogenous NO system should play a role in the regulation of AQP water channels in rat kidney subjected to I/R injury.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Artéria Renal
/
Reperfusão
/
Western Blotting
/
Constrição
/
NG-Nitroarginina Metil Éster
/
Aquaporinas
/
Isquemia
/
Rim
/
Óxido Nítrico
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Nephrology
Ano de publicação:
2006
Tipo de documento:
Artigo
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