Study of Genomic Heterogeneity of Hepatocellular Carcinomas by Using Arbitrary Primer Polymerase Chain Reaction (AP-PCR) / 한국간담췌외과학회지
Korean Journal of Hepato-Biliary-Pancreatic Surgery
; : 26-32, 2002.
Article
em Ko
| WPRIM
| ID: wpr-89472
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/AIMS: Discrimination between intrahepatic metastasis (IM) and de novo multicentric occurrence (MO) in multiple hepatocellular carcinoma (HCC) is important not only for the study of hepatocarcinogenesis but also for determination of therapeutic strategies. The purpose of this study is to evaluate the clonality of multiple or recurrent hepatocelluar carcinoma by using AP-PCR. METHODS: Paraffin-embedded blocks of 9 multiple synchronous hepatocellular carcinomas, one recurrent hepatocellular carcinoma and one combined hepatocellular carcinoma and intrahepatic cholangiocarcinoma were used. None of the tumors was larger than 3.3 cm in diameter. Microdissection was done by using sterile 27 gauge needles and microscope. Two different arbitrary primers (AR3, ZF3) were utilized in AP- PCR. The clonality of tumor was assessed by DNA band pattern (DNA fingerprint) of PCR product. RESULTS: Eight of nine multiple synchronous hepatocellular carcinomas had distinctly different DNA fingerprints. One recurrent hepatocellular carcinoma and one combined hepatocellular carcinoma and intrahepatic cholangiocarcinoma also had different DNA fingerprints. CONCLUSION: AP-PCR is a simple and very powerful method for determining the clonality of multiple hepatocellular carcinomas. The majority of multiple, small-sized hepatocellular carcinomas have different clonalities and it seems that a significant number of multiple hepatocellular carcinomas are of multicentric, de novo nature.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Características da População
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DNA
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Reação em Cadeia da Polimerase
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Impressões Digitais de DNA
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Colangiocarcinoma
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Carcinoma Hepatocelular
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Microdissecção
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Discriminação Psicológica
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Agulhas
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Metástase Neoplásica
Tipo de estudo:
Clinical_trials
Idioma:
Ko
Revista:
Korean Journal of Hepato-Biliary-Pancreatic Surgery
Ano de publicação:
2002
Tipo de documento:
Article