Distinct Inflammatory Profiles in Atopic and Nonatopic Patients With Chronic Rhinosinustis Accompanied by Nasal Polyps in Western China
Allergy, Asthma & Immunology Research
;
: 346-358, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-89606
ABSTRACT
PURPOSE:
The role of systemic sensitization in the pathophysiology of chronic rhinosinusitis with nasal polyps (CRSwNP) remains elusive. This study sought to characterize the pattern of cytokines in polyp tissues from atopic and nonatopic patients with CRSwNP.METHODS:
Atopic and nonatopic polyp and normal tissues were collected from 70 CRSwNP patients and 26 control subjects, respectively. The distribution of inflammatory cells (eosinophils, neutrophils, mast cells, etc.) were examined using immunohistochemistry, the mRNA levels of the transcription factors GATA-3, T-bet, RORc, and FOXP3 were determined using quantitative real-time polymerase chain reaction. The levels of inflammatory mediators (IFN-gamma, IL-5, IL-17A, etc.) in tissue homogenates were measured using enzyme-linked immunosorbent assay (ELISA). Moreover, the levels of inflammatory mediators in the supernatant of anti-IgE stimulated polyp tissues were measured using ELISA.RESULTS:
Atopic CRSwNP patients were characterized by increased eosinophil accumulation, enhanced eosinophilic inflammation (elevated IL-5, ECP, and total IgE), and significantly increased GATA-3 mRNA levels (P<0.05), whereas both atopic and non-atopic CRSwNP patients showed decreased FOXP3 mRNA expression (P<0.05). After addition of anti-IgE stimulation, atopic CRSwNP patients produced more IL-5, IL-2, IL-10, IL-17A, and PGD2 in the supernatant of stimulated polyp tissues than nonatopic CRSwNP patients did.CONCLUSIONS:
Atopic and nonatopic CRSwNP patients may possess the patterns of inflammatory response in polyp tissues.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pólipos
/
Fatores de Transcrição
/
Imunoglobulina E
/
RNA Mensageiro
/
Ensaio de Imunoadsorção Enzimática
/
Imuno-Histoquímica
/
Prostaglandina D2
/
China
/
Pólipos Nasais
/
Citocinas
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Allergy, Asthma & Immunology Research
Ano de publicação:
2015
Tipo de documento:
Artigo
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