Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter
Experimental & Molecular Medicine
;
: 101-110, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-90142
ABSTRACT
In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-gamma in the liver. Rosiglitazone, PPAR-gamma agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-gamma/RXR-alpha. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-gamma rather than PPAR-alpha binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-gamma ligand contributing to glucose transport into liver in a condition when PAPR-gamma expression is increased as in type 2 diabetes or in severe obesity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas de Transporte de Monossacarídeos
/
Camundongos Transgênicos
/
Células Cultivadas
/
Regulação da Expressão Gênica
/
Mutagênese Sítio-Dirigida
/
Regiões Promotoras Genéticas
/
Genes Reporter
/
Isoformas de Proteínas
/
Elementos de Resposta
/
Hepatócitos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo
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