Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip
Journal of Veterinary Science
;
: e4-2020.
Artigo
em Inglês
| WPRIM
| ID: wpr-901420
ABSTRACT
Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2020
Tipo de documento:
Artigo
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