Expression of Bcl-2 Protein in Ischemia-Reperfused Myocardium of Rabbit / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 924-927, 1998.
Artigo
em Coreano
| WPRIM
| ID: wpr-90399
ABSTRACT
BACKGROUND:
Myocardial cell death after myocardial infarction or reperfusion is classified into necrosis and apoptosis. Bcl-2 protein is a cytoplasmic protein, which inhibits apoptosis and is expressed in acute stage of myocardial infarction but not in normal heart. This study was performed to investigate whether Bcl-2 protein was expressed respectively to the reperfusion time. MATERIALS ANDMETHODS:
Thirty nine New Zealand white rabbits weighing 1.5-4.8 kg (mean, 2.9kg) were alloted into 7 groups (n=5 in each group) which underwent left anterior descending coronary artery (LAD) occlusion for 30 minutes, followed by reperfusion. The animals were sacrificed at 1, 4, 8, 12, 24 hours, and 3, 7 days after occlusion. Ventricle was excised immediately after intervention. Tissues were fixed in 10% buffured formalin and embedded in paraffin. Bcl-2 protein was detected by immunohistochemical stain with using monoclonal antibody against Bcl-2 protein.RESULTS:
The positive immunohistochemical reactivity for Bcl-2 protein was observed in 12, 24 hours, and 3 days reperfusion groups. Bcl-2 protein was detected in salvaged myocytes surrounding the infarcted area.CONCLUSIONS:
Bcl-2 protein is expressed at the late acute stage of infarct. Therefore, the expression of Bcl-2 protein may not protect acute cell death, but may play a role in the prevention of late cell death after myocardial is chemia-reperfusion.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parafina
/
Reperfusão Miocárdica
/
Reperfusão
/
Morte Celular
/
Apoptose
/
Vasos Coronários
/
Citoplasma
/
Células Musculares
/
Formaldeído
/
Coração
Limite:
Animais
Idioma:
Coreano
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
1998
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS