Screening of Qualitative Preparation Quality Markers of Yuliantang / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 101-109, 2021.
Artigo
em Chinês
| WPRIM
| ID: wpr-905839
ABSTRACT
Objective:
To screen qualitative preparation quality markers of Yuliantang, in order to provide data support for the selection of indicator components, and establish the direct connection between indicator components and efficacy (Xiehuo Zhitong) for achieving the quantity-effect combination.Method:
The stability of preparation process of Yuliantang lyophilized powder was investigated by HPLC fingerprint technology, then, the components in Yuliantang lyophilized powder were identified by UHPLC-LTQ-Orbitrap-MS. By referring to the relevant literature, the pharmacological activities of these identified compounds were compared with the pharmacological effects corresponding to the efficacy of Yuliantang, and the composition of the qualitative preparation quality markers of Yuliantang lyophilized powder was determined.Result:
The similarities between HPLC fingerprint of 10 batches of Yuliantang lyophilized powder and the control fingerprint were >0.9, indicating that the preparation process was stable and feasible. A total of 29 components were identified from Yuliantang, of which 23 alkaloids, 3 phenylpropanoids, 2 sesquiterpenoids and 1 limonoid, and there were 15 ingredients of<italic> </italic>Coptidis Rhizoma, 12 ingredients of<italic> </italic>Euodiae Fructus, and 2 ingredients of<italic> </italic>Aucklandiae Radix. The composition of the qualitative preparation quality markers of Yuliantang was initially determined as magnoflorine or 10-hydroxy-2,3,9-trimethoxyberberine, phellodendrine, menisperine, thalifendine, groenlandicine, dehydroevodiamine, coptisine, jatrorrhizine, columbamine, methylcoptisine, berberine, epiberberine, palmatine, evodiamine, rutaecarpine, neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, limonin, costunolide, dehydrocostus lactone.Conclusion:
The method for researching and screening the preparation quality markers in Yuliantang lyophilized powder is scientific, reasonable and feasible, it can provide reference for the determination of component indicators in the process research of Yuliantang and qualitative and quantitative indexes in its quality standard.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Pesquisa qualitativa
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Chinese Journal of Experimental Traditional Medical Formulae
Ano de publicação:
2021
Tipo de documento:
Artigo
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