Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN-induced matrix metalloproteinase-9 expression
Experimental & Molecular Medicine
;
: 239-245, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-90608
ABSTRACT
Unmethylated CpG oligodeoxynucleotides (CpG ODNs) activate immune cells to produce immune mediators. This study demonstrates that in murine macrophage RAW 264.7 cells, CpG ODN-mediated matrix metalloproteinase-9 (MMP-9) expression is regulated at transcriptional level and requires de novo protein synthesis. Inhibition of ERK and p38 MAPK, but not JNK, results in significant decrease of CpG ODN-induced MMP-9 expression. We found that endosomal maturation inhibitors, chloroquine and bafilomycin A, block CpG ODN-induced ERK and p38 MAPK activation and the subsequent MMP-9 expression. We also observed that CpG ODN induces NF-kappa B activation and NF-kappa B is a downstream target of p38 MAPK. Taken together, our data demonstrate that CpG ODN triggers MMP-9 expression via TLR-9 dependent ERK and p38 MAPK activation followed by NF-kappa B activation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligodesoxirribonucleotídeos
/
Transdução de Sinais
/
Linhagem Celular
/
Indução Enzimática
/
NF-kappa B
/
Proteína Quinase 1 Ativada por Mitógeno
/
Metaloproteinase 9 da Matriz
/
Proteínas Quinases Ativadas por Mitógeno
/
Proteína Quinase 3 Ativada por Mitógeno
/
Proteínas Quinases p38 Ativadas por Mitógeno
Limite:
Animais
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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