Toxicity and Efficacy of Water-eluted Fraction from Siegesbeckiae Herba Against Human Embryonic Lung Fibroblasts / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 76-82, 2021.
Artigo
em Chinês
| WPRIM
| ID: wpr-906425
ABSTRACT
Objective:
To detect the toxicity of water-eluted fraction from Siegesbeckiae Herba (SWEF) at different concentrations against MRC-5 human embryonic lung fibroblasts and its impacts on the expression of <italic>α</italic>7 nicotinic acetylcholine receptor (<italic>α</italic>7nAChR) and inflammatory factors, so as to figure out the active components responsible for toxicity and efficacy.Method:
The toxicities of SWEF at 1, 6, 10, 20, and 50 g·L<sup>-1</sup> against MRC-5 cells were determined by cell counting kit-8 (CCK-8) assay combined with flow cytometry and Trypan blue staining. The changes in <italic>α</italic>7nAChR expression and inflammatory factor levels before and after <italic>α</italic>7nAChR gene silencing were detected to reveal the pharmacodynamic effect of SWEF on MRC-5 cells.Result:
SWEF (≥6 g·L<sup>-1</sup>) obviously inhibited the viability of MRC-5 cells (<italic>P</italic><0.01) and promoted their apoptosis and necrosis (<italic>P</italic><0.01), with the half-maximal inhibitory concentration (IC<sub>50</sub>) being 6.03 g·L<sup>-1</sup>. The determination of <italic>α</italic>7nAChR expression and inflammatory factor levels in MRC-5 cells showed that SWEF contained <italic>α</italic>7nAChR agonist-like substance, which enhanced <italic>α</italic>7nAChR mRNA and protein expression (<italic>P</italic><0.05, <italic>P</italic><0.01) and decreased the inflammatory factor levels (<italic>P</italic><0.05, <italic>P</italic><0.01). SWEF down-regulated the inflammatory factors possibly by re-regulating <italic>α</italic>7nAChR mRNA expression, exhibiting a negative correlation between them (<italic>P</italic><0.01).Conclusion:
SWEF (≥6 g·L<sup>-1</sup>) is highly toxic to MRC-5 cells. Pharmacodynamic studies have confirmed that <italic>α</italic>7nAChR agonist-like substance contained in SWEF was responsible for the elevated <italic>α</italic>7nAChR expression and reduced inflammatory cytokines. It is inferred that excessive <italic>α</italic>7nAChR agonist-like substance may trigger the toxicity of<italic> </italic>Siegesbeckiae Herba.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Experimental Traditional Medical Formulae
Ano de publicação:
2021
Tipo de documento:
Artigo
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