Isolation and identification of Murine Cerebral Microglial Cells
Journal of Korean Neurosurgical Society
; : 428-441, 1992.
Article
em Ko
| WPRIM
| ID: wpr-90712
Biblioteca responsável:
WPRO
ABSTRACT
Even though the brain has been considered to be an immunologically privileged organ, recent reports showed that certain cells of the brain may be involved in immunological process in the brain. For example, some cells of the brain can present antigen to T-lymphocytes, to express class II major histocompativility antigen, and secrete interleukin-1 and -3 molecules. In addition, they are capable to phagocytose particles and possess receptors for the Fc portion on IgG. In this study, the authors tried to isolate the microglial cells from new born mice and characterize them. The isolated cells could produce such reactive oxygen intermediates(ROIs) an superoxide and hydrogen peroxide that were measured by luminometer after amplification by lucigenin and luminol respectively and could secrete reactive nitrogen intermediates(RNIs), when the cells were incubated with r-IFN plus LPS. The cells could also ingest fluorescent particles and raise intracellular calcium after stimulation with agonists when measured by flow cytometer. Our data showed that the microglial cells of the brain may belong to a member of mononuclear phagocytic system(MPS) of the body that are responsible for the host defence against invading microorganisms.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oxigênio
/
Encéfalo
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Imunoglobulina G
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Linfócitos T
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Cálcio
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Interleucina-1
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Superóxidos
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Peróxido de Hidrogênio
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Luminol
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Nitrogênio
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
Ko
Revista:
Journal of Korean Neurosurgical Society
Ano de publicação:
1992
Tipo de documento:
Article