Effects of Huashenzilu on chronic eczema in mice model / 中国临床药理学与治疗学

ABSTRACT

AIM:To observe clinical the effects of Huashenzilu on mice models with acute and chronic eczema.METHODS:Eczema was induced by repeated local exposure to DNCB,and then the mice received Huashenzilu.Using ELISA kit method to test the serum concentration of IL-2,IL-4,TNF-α,IFN-γ,and HE staining to test histopathological changes.The ear swelling and scratching index were measured,and ear allergy score were measured according to the standard for evaluation.RESULTS:After the treatment of Huashenzilu,the serum concentration of IL-4,TNF-α significantly increased and the serum concentration of IL-2,IFN-γ significantly decreased in the chronic mice compared to the model group (P ＜ 0.01).The ear swelling,scratching index and ear allergy score were significantly decreased.The phenomenon of epidermal thickening,acanthosis layer cavitation,and mast cells,macrophages infiltration were improved compared to the model group.CONCLUSION:Huashenzilu can control the chronic eczema in mice through the mechanism of regulating the balance between Th1 and Th2 immune response.